Figure 6
Mø-IL-25 promotes the expression of enzymes responsible for lipolysis and the translocation and oxidation of lipids. For adoptive transfer, BMMCs stimulated with or without IL-25, IL-15 and IFNγ for 2 days were collected in cold PBS, and 1 × 106 cells were adoptively transferred into every mouse intraperitoneally at the end of the HFD program. The serum and eWAT tissues were collected to perform the measurements 2 days after the adoptive transfer of activated macrophages. The data are presented as the mean±s.d. n=5; *P<0.05 versus the NCD or HFD group; #P<0.05 versus the HFD-Mø group. (a) The lipid level in the serum after the adoptive transfer of Mø-IL-25, Mø-IL-15 and Mø-IFNγ. (b) The level of HDL and LDL/VLDL lipoproteins in the serum after adoptive transfer. (c) The mRNA expression of Atgl in the eWAT. (d) The mRNA expression of Cd36 in the eWAT. (e) The mRNA expression of Cpt1α in the eWAT. (f) The mRNA expression of Cidea in the eWAT. BMMC, bone marrow-derived macrophage; eWAT, epididymal white adipose tissue; HFD, high-fat diet; IFN, interferon; IL, interleukin; NCD, normal chow diet; PBS, phosphate-buffered saline.
