Abstract
Epidermal growth factor (EGF) induced intracellular free calcium ([Ca2+]i) response was studied in fura-2- or fluo-3-loaded human hepatoma cells of BEL-7404 cell line. Single cell [Ca2+]i analysis and [Ca2+]i measurement in cell populations revealed that EGF triggered a rapid [Ca2+]i increase in the dose-dependent and time- dependent manner. Pretreatment of cells with an endoplasmic reticulum (ER) Ca2+-ATPase inhibitor, thapsigargin (TG) at 100 nM concentration for 20 min, completely abolished EGF-induced [Ca2+]i increase, and chelating extracellular calcium by excess EGTA partially inhibited the increase. Furthermore, the expression of antisense EGF receptor sequence in BEL-7404 cells suppressed the [Ca2+]i response to EGF. The results suggest that EGF receptor-mediated [Ca 2+]i increase in the human hepatoma ceils is essentially dependent on the Ca2+ storage in ER.
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*The project is supported by China Postdoctoral Science Foundation and Shanghai Joint Laboratory of Life Science Foundation
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Fu, T., Xu, Y., Jiang, W. et al. EGF receptor-mediated intracellular calcium increase in human hepatoma BEL-7404 cells. Cell Res 4, 145–153 (1994). https://doi.org/10.1038/cr.1994.15
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DOI: https://doi.org/10.1038/cr.1994.15
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