Abstract
Clear G-bands were revealed by applying the TUG method on chromosomes of 5 species of higher plants (Lilium davidii, Viciafaba, Hordeum vulgare, Ginkgo biloba and Triticum monococcum). Some details of the TUG method which consisted of treating chromosomes with both trypsin and urea were also studied. The mechanisms that might account for the presence of G-bands in plants were discussed.
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References
Chen RY, Song WQ, Li XL . A new method for preparing mitotic chromosomes from plant. Acta Bot Sin 1979; 21:297–8.(in Chinese)
Chen RY, Song WQ, Li XL . Wall degradation hypotonic method of preparing chromosome sample in plant and its significance in the cytogenetics. Acat Gent Sin 1982; 9:151–9.(in Chinese)
Chen RY, An ZP, Song WQ, Li XL, Su JY . A preliminary study on the G-bands of chromosomes in some plants. J Wuhan Bot Res 1986; 4:111–7. (in Chinese)
Chen RY, An ZP, Song WQ, Li XL, Su JY, Zheng JY . A study of the high-resolution G-banding technique of plant chromosomes. Acat Bot Sin 1987; 29:341–6. (in Chinese)
Chen RY . G-band staining of chromosomes in some higher plants with special reference to Lilium davidii. La Kromosoma 1988; II- 50:1635–51.
Comings DE, Avelino E, Okada TA, Wyandt HE . The mechanism of C-and G-banding of human chromosomes. Exp Cell Res 1973; 77:469–93.
Drewry A . G-banded chromosomes in Pinus resinosa. Hered 1982; 73:305–6.
Drewry A . The G-banded karyotype of Pinus resinosa. Silvae Genet 1988; 37:218–21.
Greihuber J . Why palnt chromosomes do not show G-bands. Theor Appl Genet 1977; 50:121–4.
Mckay RDG . The mechanism of G-and C-banding in mammalian metaphase chromosomes. Chromosoma(Berlin) 1973; 44:1–4.
Rohme D . Heneen WK . Premature chromosome condensation, application in basic, clinical, and mutation Research (Ed. P. N.) Pao RT, Johnson K. Sperling 1982:131–53.
Yunis JJ . the characterization of high-resolution G-banded chromosome of man. Chromosoma(Berlin) 1978; 67:293–307.
Zhan TS, Shi LM, Hsu TC . Induction of G-bands on root of Zea mays. Acta Bot Sin 1987; 29:465–8.(in Chinese)
Zhang ZL, Yang XF . Research on G-banding of Secale cereale chromosomes. Acta Bot Sin 1986; 28:595–8.(in Chinese)
Zhu FS, Fu JH, Li LC . A preliminary study of high-resolution G-banded chromosomes of Hordeum vulgare. Acta Agri Sin 1986; 12:213–4.
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*The project was supported by the National Natural Sciences Foundation of China.
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Chen, R., Song, W., Li, X. et al. Chromosome G-banding in plants by inducing with trypsin and urea. Cell Res 4, 79–87 (1994). https://doi.org/10.1038/cr.1994.8
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DOI: https://doi.org/10.1038/cr.1994.8
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