Abstract
A 6kb rDNA probe comprising the 18S coding plus spacer sequences has been hybridized to the metaphase chromosomes of apple rootstock cultivar MM106 demonstrating the localization of ribosomal gene arrays in the vicinity of the telomeric regions of the short arms of chromosomes 6 and 14. The in situ results using digoxygenin labelling coupled to an alkaline phosphatase immunoassay were confirmed by silver staining for NORs and nucleoli. This study demonstrates the feasibility of molecular cytogenetic analysis of very small chromosomes(1.0–2.7μm) of apple.
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This research was funded by the New Zealand Foundation for Research, Science and Technology, Contract Number C06209.
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*Dedicated to Professor Zhen YAO.
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Zhu, J., Gardiner, S. Chromosomal localization of ribosomal DNA sequences in an apple rootstock using a digoxygenin detection system. Cell Res 5, 1–7 (1995). https://doi.org/10.1038/cr.1995.1
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DOI: https://doi.org/10.1038/cr.1995.1
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