Abstract
Using laser scanning confocal microscopy, we have found that the in cells loaded with fluo-3/ AM, highest intracellular Ca2+ in the perinuclear region is associated with the Golgi apparatus. The spatiotemporal subcellular distribution of Ca2+ in living human fibroblasts exposing to calcium-free medium in response to agonists has been investigated. PDGF, which releases Ca2+ from intra-cellular stores by inositol(1, 4, 5)-trisphosphate pathway, produced a biphasic transient rise in intracellular calcium. The initial rise was resulted from a direct release of calcium from the Golgi apparatus. Calcium could be also released from and reaccumulated into the Golgi apparatus by the stimulation of thapsigargin, an inhibitor of the Ca2+ transport ATPase of intracellular calcium store. Permeablizing the plasma membrane by 10 μM digitonin resulted in the calcium release from the Golgi apparatus and depletion of the internal calcium store. These results suggest that the Golgi apparatus plays a role in Ca2+ regulation in signal transduction.
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Acknowledgements
We thank Dr Peter K Hepler (Biology Department, University of Massachusetts) for critical reading and for his useful comments on this manuscript. We also thank Dr HQ Zhang and M Fang for preparing the photographs. We are grateful to Meridian Instruments Inc for providing available laser scanning confocal microscope and technical help. This work was supported by grants from the National Education Committee Doctor's Foundation of China and grants from the National Natural Science Foundation of China.
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*The work is specifically dedicated to Professor Zhen YAO for his 80-years birthday
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Cui, J., Li, Y. & Xue, S. Visualization of Golgia apparatus as an intracellular calcium store by laser scanning confocal microscope. Cell Res 5, 165–179 (1995). https://doi.org/10.1038/cr.1995.16
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DOI: https://doi.org/10.1038/cr.1995.16
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