Abstract
Both calcium ionophore A23187 and endoplasmic reticulum Ca2+ - ATPase inhibitor thapsigargin (Tg) could increase intracellular free calcium concentration and induce apoptosis in some cell lines. In the present study, we found that HL-60 cells treated with A23187 (1 μg/ml) for 4 h or with Tg (0.5 μg/ml) for 2 h showed typical characteristics of apoptosis. Pretreatment with nontoxic concentration of cyclosporin A (CsA) (1 μg/ml) could block these effects. Flow cytometric analysis of intracellular Ca2+ after staining with fluo-3 AM showed that CsA did not prevent the increase of intracellular calcium induced by A23187 or Tg, but it could maintain the high level of intracellular Ca2+ for a long time. These results suggest that CsA may prevent calcium-induced apoptosis by blocking the transportation of Ca2+ in HL-60 cells.
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Acknowledgements
We thank Drs. Chi Xu Sheng and Pang Da Ban (Institute of Basic Medicine, Academy of Chinese Tradition Medicine) for measuring DNA and calcium with FCM.
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Project supported by the Natural Foundation of China and the Foundation for Ph. D. training from State Education Committee of China
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Huang, Q., Fang, M., Zhang, H. et al. Correlation between inhibition of calcium-dependent apoptosis by cyclosporin A and calcium transportation in HL-60 cells. Cell Res 6, 23–30 (1996). https://doi.org/10.1038/cr.1996.3
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DOI: https://doi.org/10.1038/cr.1996.3
