Abstract
Recently the antichromosome antisera from several scleroderma patients have been found to recognize the pellicle of metaphase and anaphase chromosomes. In order to identify the pellicle components, we used these antichromosome antisera to screen a human embryonic cDNA library. The sequences of the positive clones are identical to the cDNA gene sequence of CENP-C (centromere protein C), a human centromere autoantigen. This result suggusts that CENP-C is a component of the pellicle of human metaphase and anaphase chromosomes.
Similar content being viewed by others
Log in or create a free account to read this content
Gain free access to this article, as well as selected content from this journal and more on nature.com
or
References
Bloom K . The centromere frontier: kinetochore components, microtubule-based motility, and the CEN-value paradox. Cell 1993; 73:621–24.
Saitoh H, Tomkiel J, Cooke CA, Ratrie III H, Maurer M, Rothfield NF, Earnshaw WC . CENP-C, an autoantigen in scleroderma, is a component of the human inner kinetochore plate. Cell 1992; 70:115–25.
Tomkiel J, Cooke CA, Saitoh H, Bernat RL, Earnshaw WC . CENP-C is required for maintaining proper kinetochore size and for a timely transition to anaphase. JCB 1994; 125:531–45.
Tsu TC, Arriglui FE, Klevecz RR et al. The nucleolus in mitotic divisions of mammalian cells in vitro. J Cell Biol 1965; 26:539–53.
Shi Luji ( L. C. Sze ), Ni Zumei, Zhao Shi et al. Involvement of a nucleolar component, perichromonucleolin, in the condensation and decondensation of chromosomes. PNAS 1987; 87:7953–6.
Ni Zumei, Je Little, Shi Luji ( L. C. Sze ). Assembly and disassembly of mammalian chromosome pellicle. Cell Research 1992; 2:195–201.
Ni Zumei, Su lide, Shi Luji ( L. C. Sze ) et al. The Localization of antisera of scleroderma patients and their antigen in cell structure. J of Chinese Medicine 1993; 73:270–3.
Wei Ying, Ni Zumei, Zhou Yan, Wu Li, Shi Luji ( L. C. Sze ). The study of metaphasic chromosome pellicle. Acta Biologiae Experimentalis Sinica 1994; 27:505–13.
Sambrook J, Fritsch EF, Maniatis T . Molecular Cloning: a Laboratory Manual. 2nd Edition, Cold Spring Harbor Laboratory Press, Cold Spring Habor, New York. 1989.
Acknowledgements
This research was supported by the State High Tech Development Program (863) Foandation of China and RGC Research Grant of Hong Kong.
Author information
Authors and Affiliations
Corresponding author
Additional information
*This article is dedicated to Dr. Lu Ji SHI (L. C. Sze) in honor of his 80th birthday.
Rights and permissions
About this article
Cite this article
Xie, Y., Ni, Z., Gu, J. et al. Human chromosome pellicle antibody recognizing centromere protein-C (CENP-C), the main component of the kinetochore. Cell Res 7, 13–19 (1997). https://doi.org/10.1038/cr.1997.2
Received:
Revised:
Accepted:
Issue date:
DOI: https://doi.org/10.1038/cr.1997.2
