Abstract
Human gastric cancer MKN-45 cells were transfected with pULB 3238, a plasmid carrying MVMp NS-1 gene with its original P4 promoter replaced by the glucocorticoid inducible promoter MMTV-LTR. After the integration and expression of NS-1 gene, some of the transfectants died, while others remained alive, but the growth features of survived cells were changed. For further study on the antineoplastic function of parvoviral NS-1 protein in vivo, transgenic mice carrying NS-1 genes were established by conventional method. Among 4 founders, one of them was found to be able to transmit the transgene to around 50% of their offsprings. RT-PCR was performed to indicate the expression of NS-1 gene in transgenic mice and its mRNA appeared in a variety of tissues. The expression of integrated NS-1 gene may correlate with the decreased incidence of tumor induced in vivo by chemical carcinogens.
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Acknowledgements
We thank Dr J. Rommelaere for plasmid pULB 3238, Professor Luo ZY for plasmid pSV2neo. We are indebted to Xu GJ for help in maintaining lines of transgenic mice. This work was supported by National Natural Science Foundation of China(NO. 39270764, NO. 39570782).
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Shen, X., Tsung, H., Yang, Y. et al. A preliminary analysis of antineoplastic activity of parvovirus MVMp NS-1 proteins. Cell Res 7, 217–227 (1997). https://doi.org/10.1038/cr.1997.22
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DOI: https://doi.org/10.1038/cr.1997.22
