Abstract
The human norepinephrine transporter(NET) gene was cloned and structurally analyzed. The far 5' fragment containing exon 1 (a non-coding exon) and exon 2 was sequenced. The transcription start site of the gene in human brain stem tissue was determined by primer extension analysis. It was found that the gene could be transcribed from multiple starting points. The 5' flanking sequence contains a proximal G-C rich region, one possible GSG element and several SP1 sites. However it does not contain TATA box and CAAT box motifs. Gel shift analysis with nuclear extracts from different tissues of mouse shows that the G-C rich region may be involved in tissue specific expression of the gene.
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Acknowledgements
We thank Professor WANG Da, Dr. XIA Guo Hong and Dr. YIN Guo Yong for their kind suggestion and help. This work was supported by Foundation From Chinese Academy of Sciences and Qi Ming Xing Foundation from Shanghai Science and Technology Committee.
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Huang, F., Fei, J., Ma, S. et al. Analysis of the 5' flanking sequence of the human norepinephrine transporter gene. Cell Res 8, 143–149 (1998). https://doi.org/10.1038/cr.1998.14
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DOI: https://doi.org/10.1038/cr.1998.14
