Figure 4
From: Lineage-specific distribution of high levels of genomic
5-Hydroxymethylcytosine (5-hmC) localises with nestin-expressing stem cell populations in 12.5 dpc mouse embryos. (A) Sagital section of 12.5 dpc mouse embryo with DNA visualised and B, C, D, E and F views indicated with dotted squares. Immunohistochemical detection of 5-hmC in sagital sections of mouse embryonic brain (B, C) and heart (D). Localisation of 5-hmC and nestin in the sagital adjacent sections of tailbud (E) and a part of the spinal cord (F). DAPI signal in nestin immunostaining was colourised as red. Anti-single-stranded DNA antibody was used for DNA visualisation in 5-hmC immunostaining experiments. Scale bars are 150 μm in B, C and D and 300 μm in E and F. The experiments were performed using the Diagenode antibody.
