Figure 1
From: The human long non-coding RNA-RoR is a p53 repressor in response to DNA damage
RoR suppresses the doxo-induced p53 and the p53-mediated cellular function. (A) Detection of p53 in MCF-7 cells with or without doxo. Cells were transfected with vector or RoR and then treated with doxo at 1 μg/ml for 24 h. Total cellular protein was then extracted for western blot. (B) Suppression of RoR by RNAi induces p53. MCF-7 and HCT-116 WT cells were transfected with control siRNA or RoR-siRNA-1 or RoR-siRNA-2. (C) RoR inhibits the doxo-induced apoptosis. The transfected MCF-7 cells were treated with doxo at 2 μg/ml for 24 h before TUNEL assay was performed. A high concentration of doxo was used to detect cell apoptosis. (D) RoR suppresses the doxo-induced G2/M arrest. HCT-116 WT cells were transfected with vector or RoR, and then treated with doxo at 0.1 μg/ml for 24 h, followed by FACS cell cycle analysis. A low concentration of doxo was used to detect cell cycle arrest.
