Figure 3
From: β-Arrestin1 regulates γ-secretase complex assembly and modulates amyloid-β pathology
Interference of β-arrestin1/APH-1 interaction reduces Aβ production. (A) Schematic plot of the TAT-tagged peptides corresponding to the cytoplasmic loops and C-terminus of APH1-AL and APH1-B. (B, C) ACT or BCT treatment blocked the interaction between β-arrestin1 and APH1-AL (B), or APH1-B (C). HEK293T cells overexpressing HA-tagged β-arrestin1 and Flag-tagged APH1-AL or APH1-B were treated with 10 μM of the indicated TAT-tagged peptides in the media for 36 h. Mock represented the sample treated by media. Triton X-100 extracts were subjected to Co-IP assay. (D) APPswe HEK293 cells were treated with 0, 1 or 10 μM of the indicated TAT-tagged peptides in the media for 36 h. Media were then collected and subjected to ELISA specific for Aβ. Error bars represent SEM. (E) Notch processing is not affected by TAT-tagged peptides treatment. HEK293 cells overexpressing Myc-tagged NotchΔE (NΔE) were treated with 10 μM of the indicated TAT-tagged peptides. Cell lysates were then subjected to SDS-PAGE, immunoblotted by the indicated antibodies.
