Figure 1
From: A distinct response to endogenous DNA damage in the development of Nbs1-deficient cortical neurons
Nbs1 deficiency leads to microcephaly. (A) Macroscopic view of reduced cerebral size in NbnCNS-del brain, n = 20, P < 0.01 (t test). (B) NeuN staining of coronal section of the cerebrum at postnatal day 21 (P21), original magnification ×1. Cx, cerebral cortex; CC, corpus callosum; H, hippocampus; fc, fasciole cinerum; Mhb, medial habenular neurons; T, thalamic region; HT, hypothalamic region. (C) Representative laminar cortical structure in control and NbnCNS-del mice. (D) Histogram showing the percentage of neurons in each layer of NbnCNS-del cerebral cortical sections compared to control mice. At least 10 mice of each genotype were analyzed, *P < 0.01 (t test). (E) Representative P7 hippocampus with NeuN staining (left). DG, dentate gyrus; CA1/CA3, field CA1/CA3 of hippocampus; PoDG, polymorph layer dentate gyrus. Bar is 1 mm. Representative MBP staining for CC and neurofibers (right). (F) Quantification of the thickness of CC in NbnCNS-del brain at the indicated site. Original magnification ×10. At least 10 mice of each genotype were analyzed, *P < 0.01 (t test).
