Figure 2
From: A distinct response to endogenous DNA damage in the development of Nbs1-deficient cortical neurons
Nbs1 deficiency impairs ATR-Chk1 signaling in response to endogenous DNA damage in developing brain. (A) Accumulation of γH2AX exists in VZ and MZ of Nbs1-deficient embryonic brain at E12.5. Original magnification ×40. (B) Schematic of the role of Nbs1 in response to DNA damage. (C) Nbs1 is required for the activation of ATR signaling during neurogenesis. Western blot analysis of the activation of ATR pathway in embryonic cortex. Note: In the absence of Nbs1, a marked reduction of Chk1-S345p at E15.5–18.5 during neurogenesis.
