Figure 3
From: A distinct response to endogenous DNA damage in the development of Nbs1-deficient cortical neurons
Nbs1 deficiency impairs phosphorylated Chk1 focus formation. Primary cultured NbnCNS-ctr and NbnCNS-del developing neurons were treated with 2 mM HU for 3 h, and subjected to immunoflurescence staining with antibodies against phosphorylated Chk1-S345p (A), BRCA1 (B), γH2AX (C), and Nbs1 partner Mre11 (D). DAPI stains nuclei. The neuroprogenitors (A-D) are verified by nestin-positive immunoactivity, and at least 150 cells per staining were analyzed.
