Figure 3

VEGF-A-treated human Isl1⁺ progenitors express EC markers and secrete angiocrine factors in a similar pattern to the human outflow tract-derived ECs. (A) FACS analyses on the purified CD144⁺CD31⁺ ECs from the human Isl1-cre eGFP⁺ cells (Isl1-ECs) in the presence of VEGF-A from day 7-21. (B, C) Correlation of angiocrine gene expression between qPCR data obtained from the day-7 purified human Isl1-cre eGFP⁺ cells (Isl1 progenitors) or Isl1-ECs in the presence of VEGF-A from day 7-21 and (B) the purified CD144⁺CD31⁺ ECs from the outflow tract of human fetal hearts at 10 weeks of gestation (OFT-ECs) or (C) the noncardiac, human cord blood-derived outgrowth endothelial cells (OECs) (n = 5).