Figure 4
From: L-glutamine provides acid resistance for Escherichia coli through enzymatic release of ammonia
The amino acid antiporter GadC is required for the Gln- and YbaS-mediated acid resistance. (A) Deletion of the gadC gene resulted in loss of acid resistance in the presence of 10 mM Gln. Shown here are results of acid resistance assays performed on WT E. coli and its variant ΔgadC. (B) Gln-dependent survival of E. coli ΔgadC under acidic environment can be rescued by transformation of a gadC-expressing plasmid. (C) Restoration of ammonium release by transformation of the gadC-expressing plasmid, but not the empty plasmid. The results shown in panels (A-C) represent the average of three independent experiments, with the SDs indicated.
