Figure 2
From: Def defines a conserved nucleolar pathway that leads p53 to proteasome-independent degradation
Def-mediated p53 degradation was independent of Mdm2. (A) Diagram on the top shows the Mdm2-binding domain and other key functional domains of the p53 protein, and shows the truncated sites for Δ113p53 and p53-P5. TAD, transactivation domain; PRR, proline-rich region; DBD, DNA-binding domain; TET, tetramerization domain; CT, C-terminus regulation domain. Numerical numbers denote the amino acid position from Met. Western blot analysis of p53, Def and Mdm2 examined the effects of Def and Mdm2 on the stability of Δ113p53 and p53-P5 proteins at 6 hpi. Commassie Blue staining, protein loading control. (B, C) Western blot of p53 and Def for analyzing the effect of Def on endogenous p53 induced by mdm2-MO at 10 hpi and 24 hpi, respectively (B). TUNEL assay for analysis of apoptosis in embryos injected with mdm2-MO alone or mdm2-MO+def mRNA mix at 24 hpi (C). st-MO morpholino, defstop mRNA and phenol red dye injections were used as the controls.
