Figure 3
From: Def defines a conserved nucleolar pathway that leads p53 to proteasome-independent degradation
Def-mediated p53 degradation is independent of the ubiquitination pathway. (A) Diagram shows the structure of p53 protein (refer to Figure 2A) and six p53 deletion mutant constructs. (B) Western blot of p53, six p53 deletion mutant products and Def for analyzing the effect of Def on their stability in the co-injected embryos at 6 hpi. (C) Same as in (B) but analyzing the effect of Mdm2. (D) Western blot of p53, Def and Mdm2 for comparing the effect of Def and Mdm2 on the stability of p53 mutant proteins R143H, R250W and R313C in the injected embryos at 6 hpi. (E) Western blot of p53 for comparing the effect of different dosages of ubiquitin on the stability of p53 in the injected embryos at 6 hpi. (F) Western blot of p53 and Mdm2 for analyzing the effect of Mdm2 plus ubiquitin on the stability of p53 mutant protein p53-9KR in the injected embryos at 6 hpi. (G) Same as in (F) but analyzing the effect of Def.
