Figure 1
From: Subepicardial endothelial cells invade the embryonic ventricle wall to form coronary arteries
Subepicardial and intramyocardial vessels in developing heart. (A) Coronary vessel coverage over the surface of the heart from E11.5 to E14.5 (n = 9-13). Graph calculates area encircled by dotted lines. (B) PECAM staining of E10.5 to E15.5 hearts showing that subepicardial coronary vessels (arrows) develop before intramyocardial vessels (arrowheads). Right panels are boxed regions and results are representative of 5-10 hearts per time point. (C) Quantification of the number of PECAM+ subepicardial and intramyocardial coronary ECs in the left ventricle from E10.5 to E15.5. (D) Continuity analysis by quantifying the number of PECAM+ cells within 20 μm of endocardial (Endo.) or subepicardial (Subepi.) ECs at E11.5 to E14.5. *P < 0.05; n = 6-10. White bar = 0.5 mm; black bar = 200 μm; yellow bar = 50 μm.
