Figure 3
Existence of asAmp transcripts in higher eukaryotic cells. (A) PCR analysis of genomic DNAs from various human cell lines. Primer sets asAmp-F/asAmp-R, RP-F/asAmp-R, A1C7-F/A1C7-R and Kanr-F/Kanr-R are the same as those in Figure 2E. Primer set gyrB-F/gyrB-R is complementary to the sequence of bacterial gyrB cDNA. NC, negative-control without DNA templates. (B) RT-PCR analysis of asAmp transcripts in human cell lines HEK293T and THP-1. Primer set asAmp-F/asAmp-R was used. NC, without RNA templates. (C) The PCR products from HEK293T cells (B) were subjected to DNA sequencing. The sequence matching the 267-bp asAmp ORF is shown. (D, E) Alignment analysis of the asAmp ORF in EST database. The 267-bp asAmp ORF sequence is entered as query sequence by using BLASTN. (D) Results are shown by taxonomy tree with hit numbers in each species indicated in brackets. (E) The species containing the 267-bp asAmp ORF (gray bar) with 100% identity. Branch lengths are not proportional to phylogeny time.
