Figure 1
From: Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes
Derivation of monkey PG-haESCs. (A) Representative images of different stages of monkey ESC line derivation. PB, polar body; PN, pronucleus; ICM, inner cell mass. Scale bar, 100 μm (top, middle panel and bottom left) and 500 μm (bottom right). (B) Establishment of PG-haESC line (represented by MPH1) after three times of FACS enrichment for haploid cells. Right is FACS data of diploid control ESCs for comparison. D62, 62 days after the ICM was plated for ESC formation. (C) Karyotype of MPH1 (passage 7) and MPH2 (passage 5) showing the normal haploid complement of 21 chromosomes (20 + X). (D) CGH analysis of PG-haESCs (MPH1, passage 11) and female monkey adipose cells. Upper panel, MPH1 (p11) vs adipose cells. Lower panel, adipose cells vs adipose cells. No major genomic alterations (amplifications or losses) were detected in monkey PG-haESCs.
