Figure 3
From: Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes
Cellular origin of MPH1 and MPH2. (A) Representative images of STR locus (D6S501) of MPH1 and MPH2 and their egg donors (no. 183 and no. 118). (B) Representative images of SNP locus (rs5436199) of MPH1 and MPH2 and their egg donor monkeys (no. 183 and no. 118). (C) Expression of the imprinted genes measured by qPCR. The expression levels in PG-haESCs were relative to those in diploid ESCs, which were set to 1. *0.01 < P < 0.05; **0.001 < P < 0.01. (D) Methylation analysis of SNRPN and H19 in monkey skin, sperm, diploid ESCs (MES2, passage 4 for SNRPN, and passage 10 for H19) and PG-haESCs (MPH1, passage 5 for H19 and passage 7 for SNRPN; MPH2, passage 9 for H19 and passage 8 for SNRPN). Open and filled circles represent unmethylated and methylated CpG sites, respectively.
