Table 1 Derivation of monkey ESCs from parthenogenetic and ICSI-derived blastocysts
From: Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes
Treatment | Monkey number | No. of oocytes | No. of two-cell embryos | No. of compacted morulae | No. of blastocysts | No. of outgrowths | No. of established ESC lines | No. of PG-haESC lines |
|---|---|---|---|---|---|---|---|---|
Ionomycin/CHX | 128 | 5 | 3 | 3 | 1 | 0 | 0 | 0 |
Ionomycin/CHX | 183 | 6 | 6 | 6 | 3 | 1 | 1 | 1 |
Ionomycin/CHX | 147 | 5 | 5 | 5 | 0 | 0 | 0 | 0 |
Ionomycin/CHX | 212 | 5 | 1 | 0 | 0 | 0 | 0 | 0 |
Ionomycin/CHX | 97 | 5 | 5 | 5 | 2 | 0 | 0 | 0 |
Ionomycin/CHX | 154 and 164 | 23 | 23 | 22 | 11 | 5 | 4 | 0 |
Ionomycin/CHX | 152 | 14 | 11 | 11 | 5 | 0 | 0 | 0 |
Ionomycin/CHX | 95 | 7 | 4 | 4 | 0 | 0 | 0 | 0 |
Ionomycin/CHX | 69 | 7 | 7 | 2 | 0 | 0 | 0 | 0 |
Ionomycin/CHX | 26 | 15 | 15 | 15 | 9 | 4 | 0 | 0 |
Ionomycin/CHX | 45 | 5 | 4 | 4 | 2 | 0 | 0 | 0 |
Ionomycin/CHX | 102 | 9 | 9 | 9 | 4 | 0 | 0 | 0 |
Ionomycin/CHX | 27 | 3 | 3 | 3 | 2 | 0 | 0 | 0 |
Ionomycin/CHX | 161 | 30 | 30 | 30 | 11 | 5 | 0 | 0 |
Ionomycin/CHX | 191 | 1 | 1 | 1 | 1 | 0 | 0 | 0 |
Ionomycin/CHX | 197 | 1 | 1 | 1 | 0 | 0 | 0 | 0 |
Ionomycin/CHX | 115 | 6 | 6 | 6 | 4 | 0 | 0 | 0 |
Ionomycin/CHX | 227 | 4 | 4 | 2 | 1 | 1 | 1 | 0 |
Ionomycin/CHX | 118 | 28 | 27 | 24 | 13 | 5 | 4 | 1 |
Ionomycin/CHX | 14 | 1 | 1 | 1 | 0 | 0 | 0 | 0 |
Ionomycin/CHX | 211 | 1 | 1 | 1 | 1 | 0 | 0 | 0 |
Subtotal | Â | 181 | 167 | 155 | 70 | 21 | 10 | 2 |
ICSI | 5 | 15 | 15 | 7 | 4 | 2 | 1 | / |
ICSI | 87 | 3 | 3 | 3 | 1 | 1 | 0 | / |
ICSI | 183 | 10 | 8 | 4 | 2 | 1 | 0 | / |
ICSI | 195 | 9 | 4 | 3 | 2 | 1 | 0 | / |
ICSI | 207 | 7 | 6 | 4 | 4 | 3 | 0 | / |
ICSI | 50 | 20 | 13 | 10 | 10 | 10 | 3 | / |
Subtotal | Â | 64 | 49 | 31 | 23 | 18 | 4 | / |
