Figure 1

Nestin expression in the testis of Nes-GFP transgenic mice. (A) Nestin (green) expression in the testis of Nes-GFP transgenic mice on postnatal days 0, 7, 14, 28, and 90. Scale bar, 50 μm. (B) Quantification of Nes-GFP⁺ cells in the testis sections at the indicated time points. The percentage of Nes-GFP⁺ cells in the testis interstitum was counted. Three sections per slide and three slides per mouse testis were counted. (C) Immunohistochemical analyses showed that the Nes-GFP⁺ cells in the testis expressed PDGFR-α and LIFR, but did not express LHR or 3β-HSD on postnatal day 7. Nuclei were counterstained with DAPI (blue). Scale bar, 50 μm. (D) qRT-PCR analysis of Nestin, PDGFR-α and LIFR gene expression in testes of normal mice on postnatal days 7, 14, 28, 90 (the value on postnatal day 7 was defined as 1). n = 5. Data are expressed as the mean ± SEM.