Figure 2

Isolation and phenotypic analysis of Nes-GFP⁺ cells derived from the testis. (A) Flow cytometry was used to isolate Nes-GFP⁺ cells from the testes of Nes-GFP transgenic mice on postnatal days 7, 14, and 28. The representative histogram overlays showed the isotype controls (black region) and the stained samples (red lines). n = 5. (B, C) Representative flow cytometric profiles of Nes-GFP⁺ cells stained with LHR (B) and PDGFR-α (C); these cells were derived from the testes of postnatal day 7 Nes-GFP transgenic mice. FSC, Forward-scattered light; SSC, Side-scattered light; PSP, PDGFR-α single positive; DP, Nes-GFP and PDGFR-α double positive; DN, double negative; NSP, Nes-GFP⁺ single positive. (D) Phase-contrast micrographs of primary (P₀, 4 days after plating) and P₁ (7 days after plating) cells in each population derived from the PSP, DP, DN, and NSP cells. Scale bar, 50 μm. (E) Cytogenetic analysis showed that the Nes-GFP⁺ cells at P₂₅ have a diploid karyotype.