Figure 8 | Cell Research

Figure 8

From: Characterization of Nestin-positive stem Leydig cells as a potential source for the treatment of testicular Leydig cell dysfunction

Figure 8

Nes-GFP+ cells differentiated into LCs and facilitated testosterone production in the testes of 22-month-old mice after transplantation. (A) Immunofluorescence staining showed the accumulation of cells positive for PKH26 (red) and P450scc (green) or LHR (green) in the interstitial area of testes of old mice 10 days after Nes-GFP+ cell implantation. The expression levels of P450scc and LHR in the 22-month-old mice were lower than those in the young mice (3-month-old). Scale bar, 50 μm. (B) In the 22-month-old mice, the serum testosterone concentrations were significantly increased in the Nes-GFP+ cell-treated mice compared to the saline-treated old mice (*P < 0.05, **P < 0.01). n = 6. Young/Saline (+), 3-month-old mice receiving saline injection; Old/Saline (+), 22-month-old mice receiving saline injection; Old/Cells (+), 22-month-old mice receiving Nes-GFP+ cells.

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