Figure 7
From: Anp32e, a higher eukaryotic histone chaperone directs preferential recognition for H2A.Z
Anp32e and H2A.Z display similar genome-wide profiles. (A) Close to 70% of Anp32e peaks in the genome overlap with H2A.Z peaks. (B) The Anp32e and H2A.Z distribution around the TSSs of all genes. Genes were sorted according to their H2A.Z ChIP-Seq reads in HeLa cells. (C) An example region showing the co-occupancy of Anp32e and H2A.Z. (D) Differential distribution of Anp32e and H2A.Z at genes with different transcriptional activity. Genes were categorized into five groups according to their transcriptional levels. (E) RT-PCR showing relative expression levels of Anp32e in HeLa cells, HeLa cell with Anp32 knockdown and HeLa cell overexpressing Anp32e. (F) Distribution of H2A.Z around TSS regions in HeLa cells overexpressing Anp32e (left), control HeLa cells (middle) and HeLa cells with Anp32e knockdown (right). Top 40% of genes in B that display clear H2A.Z occupancy were selected for the plotting. (G) Scatter plots showing the relative change of H2A.Z densities at +1 nucleosomes and −1 nucleosomes in Anp32e-overexpressing and Anp32e knockdown HeLa cells. The density of each peak was normalized by the corresponding peak in control HeLa cells. The red lines in parallel with the diagonal line represent a difference of +0.2 or −0.2, respectively.
