Figure 1
From: Ago2 facilitates Rad51 recruitment and DNA double-strand break repair by homologous recombination
The role of diRNAs in DSB repair is restricted to repair by HR and specifically relies on Ago2. (A) HR repair rates in U2OS/DR-GFP cells treated with the indicated siRNAs. (B) HR repair was restored by incubation with 50 ng of sRNAs (0.25 ng/μl) prepared from U2OS/DR-GFP cells transfected with pCBA-I-SceI (+I) but not empty vector (−I) in Dicer-knockdown cells. (C) NHEJ repair rates in HEK 293/EJ5-GFP cells treated with the indicated siRNAs. Knockdown of Ku70 that is important for NHEJ, but not Dicer or Ago2, reduced NHEJ. (*P < 0.005, **P < 0.0001, Student's t-test). The number of GFP-positive cells was measured by flow cytometry and the repair efficiency was scored as the percentage of GFP-positive cells. The extent of repair is shown relative to the repair observed in cells treated with control siRNAs. Histograms represent the mean of three independent experiments. Data are represented as mean ± SEM.
