Figure 6
From: Acute inflammation stimulates a regenerative response in the neonatal mouse heart
The pro-proliferative activity of IL-6 on neonatal cardiomyocytes is mediated by cardiomyocyte STAT3 signaling. (A) Western blotting analyses of phospho-STAT3 (Tyr705) (p-STAT3) and STAT3 after in vitro treatment of neonatal rat ventricular cardiomyocytes with DMSO, IL-6 or both IL-6 and S3I-201 (STAT3 inhibitor). (B, C) Immunofluorescence analysis of pH3 (green) and α-actinin (red) co-localization, and Ki67 (green) and α-actinin (red) co-localization in response to IL-6 or both IL-6 and S3I-201 administration in vitro in neonatal ventricular cardiomyocytes of rats. CMs, cardiomyocytes. Scale bars, 75 μm. Values are presented as the mean ± SEM; *P < 0.05.
