Figure 1
Establishment of a eukaryotic screening system to identify PDEs in V. cholera. (A) 293T cells were transiently transfected with increasing amounts (100, 200, 300 ng; wedges) of VCA0956 and 50 ng of STING, along with IFN-β-Luc reporter. The luciferase assay was performed after 24 h. (B, C) 293T cells were transfected with increasing amounts of DncV or cGAS with or without STING, along with the IFN-β-Luc reporter. The luciferase assay (B) or immunoblot analysis of p-IRF3 (C) was performed after 24 h. GAPDH was used as the loading control. (D) VCA0956 (50 ng) was transfected into 293T cells with or without PDE VCA0785 (300 ng), along with STING (50 ng) and IFN-β-Luc reporter. Twenty-four hours later, luciferase assay was performed. Unless otherwise noted, all results in this work were representative of at least three independent experiments.
