Figure 2

miR-149 influences the tumor-promoting ability of fibroblast. (A) Schematic chart of the evaluation of gastric cancer cell line SGC-7901 cultured in different conditioned medium (CM) as indicated. (B) Effect of miR-149 on GC cell proliferation measured by the WST assay. SGC-7901 cells were cultured in the conditioned mediums from CAF transfected with either miR-149 mimics or NC mimics, or NF transfected with either anti-miR-149 or anti-NC at a final concentration of 100 nM. (C) Effect of miR-149 on GC cell proliferation evaluated by colony formation assay. SGC-7901 cells were cultured in the indicated conditioned media and the number of colonies was counted 14 days after seeding. (D) Representative images of migratory or invasive cells on the membrane (magnification 100×). (E) Average number of migratory or invasive cells counted from D. (F) Tumors derived from SGC-7901 cells mixed with different fibroblasts (CAF transfected with agomir-149 or agomir-NC, NF transfected with antagomir-149 or antagomir-NC) and injected subcutaneously into nude mice. Tumors were isolated 28 days after injection. (G) Growth kinetics of tumors in nude mice. Tumor diameters were measured and the volumes were calculated every 7 days. (H) Average weight of tumors from nude mice. (I) Representative images of tumor samples stained with Ki-67 antibody from nude mice (magnification 200×). (J) Relative proliferation index from I. All data are means ± SD of three independent experiments. *P < 0.05, **P < 0.01, student's t-test.