Figure 3
miR-149 inhibits the EMT of GC cells through IL-6. (A) Relative mRNA levels of EMT markers (E-cadherin, N-cadherin, Vimentin and Snail) in SGC-7901 cultured in the CM from CAF or NF in the presence or absence of IL-6 or IL-6 antibody as indicated were quantified by qRT-PCR. (B) Relative mRNA levels of EMT markers in SGC-7901 cultured in the CM from CAFNC or CAFmiR-149 in the presence or absence of IL-6 as indicated were quantified by qRT-PCR. (C) Protein levels of EMT markers in SGC-7901 cultured as in B were analyzed by western blotting. (D) Relative mRNA expression of EMT markers in SGC-7901 cultured in the CM from NFanti-NC or NFanti-miR-149in the presence or absence of IL-6 antibody as indicated were quantified by qRT-PCR. (E) Protein levels of EMT markers in SGC-7901 cultured as in D were detected by western blotting. (F, G) Immunofluorescence analyses were carried out in SGC7901 cultured in the CM as indicated in B or D with antibodies for E-cadherin (green), Vimentin (red), and DAPI (blue), (magnification 400×). All data are means ± SD of three independent experiments. *P < 0.05, **P < 0.01, student's t-test.
