Figure 4
Increased P2RX7 signaling in cerebral microvessel after CLP. (A) Representative images of P2RX7 (green) and ICAM-1 (red) in brain cortex after CLP. Double labeling revealed co-localization of P2RX7 and ICAM-1 in brain vessels 4 h after CLP. Scale bar, 20 μm. DAPI counterstaining indicates cell nuclei (blue). (B) Enlarged view of boxed area in A showing colocalization of P2RX7 and ICAM-1 after CLP. Scale bar, 20 μm. (C) Representative immunoblots showing P2RX7 and NLRP3 expression in microvessel extracts of sham-operated and CLP mice (mean ± SEM, n = 5). (D) Quantitative analysis of protein levels in C was performed by densitometry. Data are expressed as densitometry ratio of control. The data are expressed as percentages of values obtained from sham animals (mean ± SEM, n = 5). **P < 0.01. NS, not significant.
