Figure 7
Effect of pharmacological inhibition on brain endothelial P2RX7 cation channel currents. (A) Whole-cell patch clamp recordings from HBMEC at the holding potential of −70 mV. Histogram summaries the mean peak currents normalized to membrane capacitance detected at the given ATP concentrations. Data are expressed as means ± SEM. **P < 0.01. (B) Pharmacological evidence for the localization of P2RX7 receptors in HBMEC. 5 mM ATP was applied in the presence of P2RX7 antagonist (A438079, 10 μM). (C) Electrophysiological features of P2RX7 channel in brain endothelial cells. Increased cation channel currents by ATP application were blocked with pretreatment of A438079. (D) Effect of P2RX7 inhibition on ATP-induced activation of nonselective cation channel currents. The currents were normalized to capacitance to obtain current density (pA/pF). (E) Quantification data for D. A438079 decreased ATP-induced inward currents and outward currents (n = 11; *P < 0.05, **P < 0.01).
