Figure 7
From: A miR-130a-YAP positive feedback loop promotes organ size and tumorigenesis
bantam represses SdBP/Tgi protein level in Drosophila. (A) Alignment indicates that bantam-seed-matching sequence in SdBP/tgi 3′UTR is conserved in different species of Drosophila. Matching nucleotides were labeled in red. (B) SdBP/tgi 3′UTR sensor is repressed by bantam. Indicated sensors were transfected into Drosophila S2 cells with or without bantam for luciferase assay. Experiments were performed in duplicates. (C) bantam mimic and inhibitor effect on SdBP/Tgi protein level. S2 cells were transfected and analyzed by immunoblotting. (D) Inhibition of bantam increases SdBP/Tgi protein level in vivo. Wing discs expressing UAS-bantam.sp in the P-compartment (indicated by arrowheads) under the control of hhGal4 were subjected to immunostaining for SdBP/Tgi (red). The box in panel (I) is enlarged in panel (III). BS GFP (green) is the bantam sensor. (E) SdBP/Tgi protein level increases in bantam mutant clones in Drosophila wing discs. bantam mutant (BanΔ1) clones were indicated by negative GFP (arrowheads). SdBP/Tgi protein levels were shown by immunostaining (red). Boxes in panels (I-III) were enlarged in (I′-III′). Fly genotype: hsflp;;FRT80BubiGFP/FRT80BBanΔ1. (F) microRNA-mediated positive feedback loop of the Hippo pathway. Corresponding components in mammals and Drosophila were shown in the same color.
