Figure 1
Sensitivity and reliability of the scTrio-seq technique. (A) A flow chart illustrating the scTrio-seq technique. After a single cell was lysed with mild lysis buffer, the lysis product was centrifuged. The supernatant was transferred to a new tube for transcriptome sequencing analyses, while the pellet (containing the nucleus) was bisulfite-converted for genome (CNVs) and epigenome sequencing analyses. (B) Comparing the rate of detection of DNA segments in HepG2 scTrio-seq data and HepG2 scRRBS data. The total DNA segments are those that can be detected in the bulk HepG2 RRBS data. (C) Comparing the average DNA methylation levels of CpG sites in different genomic regions between HepG2 scTrio-seq and HepG2 scRRBS data. (D) DNA methylation pattern in gene body regions as determined from HepG2 scTrio-seq data and RRBS data. The averaged DNA methylation level of CpG sites is calculated from all RefSeq genes in regions from the TSSs to TESs and their 15-kb flanking regions. (E) Unsupervised hierarchical clustering analysis based on Pearson correlations between global CpG methylation levels of different HepG2 samples. (F) CNV deduction results at a 10-Mb resolution. The normalized copy number values (red or blue dots) for the bulk genome DNA sequencing data and bulk RRBS data are shown. For the scTrio-seq data, HMM fitting results (red or blue segments) are also shown.
