Figure 2
From: Bacteria-host relationship: ubiquitin ligases as weapons of invasion
The carboxyl terminus of NleG effectors harbors RING/U-box domain. (A) Amino acid sequence alignment of NleG family member carboxy-terminal domains as analyzed by Clustal Omega49,50. Conserved amino acids corresponding to the RING/U-box motif are denoted with asterisks. NleG family members with demonstrated Ub ligase activity in vitro are highlighted in bold italic48. (B) Comparison of RING/U-box-like domains of AvrPtoB and NleG2-3. CTD structures of AvrPtoB (PDB code: 2FD4) and NleG2-3 (PDB code: 2KKY) were superimposed using Dalilite server51. For clarity in comparison, peripheral elements of the CTDs are removed from both structures, showing only the core of the RING/U-box domains.
