Figure 1

Frequent loss of miR-1254 and CCAR1 expression in breast cancer. (A-B) Genomic qPCR of miR-1254-1 (A) and miR-4678 (B) in normal breast tissues or breast cancer tissues; the red lines indicate the threshold for gene amplification and the blue lines indicate the threshold for gene deletion. Error bar indicates standard error of the mean (SEM). ^***P< 0.001; NS, not significant. (C) List of miRNAs on chromosome 10q frequently deleted or amplified in breast carcinoma determined by genomic qPCR. miR-3944 is below the detection level. (D) Schematic diagram of the genomic locus of hsa-miR-1254-1. (E-G) Correlation between the expression levels of miR-1254 and pri-miR-1254-1 (E), CCAR1 and pri-miR-1254-1 (F) miR-1254 and CCAR1 (G) in 57 breast epithelial tissues. Error bars indicate SEM. ^***P < 0.001. (H-J) miR-1254 (H), pri-miR-1254 (I) and CCAR1 (J) expression in normal breast tissues or breast cancer tissues determined by qRT-PCR. Error bars indicate SEM. ^***P < 0.001. (K-I) Expression levels of miR-1254 (K) and CCAR1 (L) in 13 human mammary epithelial cell lines (HMECs) analyzed by qRT-PCR. Error bars indicate SEM. ^***P< 0.001. Student's t-test. See also Supplementary information, Figure S1.