Figure 2

ZIKV inhibits neural stem cell proliferation and depletes progenitor pool in dorsal ventricular zone. (A) Immunostaining for Ki67 in brain slices from P1 mice i.p. injected with ZIKV or mock control at E13.5. White dash lines indicate the boundary between VZ/SVZ and IZ zones. Scale bar, 50 μm. (B) Quantification for the number of Ki67-positive (Ki67+) cells in VZ/SVZ and IZ regions from mock and ZIKV groups. Data are shown as mean ± SEM (^*P < 0.05, ^**P< 0.01; Student's t-test). (C) Immunostaining for BLBP and Sox2 in brain slices from P1 mice i.p. injected with ZIKV or mock control at E13.5. White dash lines indicate the boundary between VZ/SVZ and IZ zones. Yellow dash lines indicate the densely arrayed continuous Sox2-positive (Sox2+) cell layers within the VZ/SVZ region. Scale bars, 50 μm. (D, E) Quantification for the band thickness of densely arrayed Sox2+ cell layers relative to that of the entire VZ/SVZ region (D) and the intensity of Sox2+ or BLBP+ cells (E). Data are mean ± SEM (^*P< 0.05, ^**P< 0.01, ^***P< 0.001, Student's t-test). (F) Immunostaining for Tbr2 in brain slices from P1 mice i.p. injected with ZIKV or mock control at E13.5. White dash lines indicate boundary between VZ/SVZ and IZ zones. Scale bars, 50 μm. (G, H) Quantification of relative intensity of Tbr2+ cells (G) and the band thickness of densely arrayed Tbr2+ cell layers relative to that of the entire VZ/SVZ region (H). Data are mean ± SEM (^**P< 0.05, ^***P< 0.001, Student's t-test).