Figure 3
From: Mettl3-mediated m6A regulates spermatogonial differentiation and meiosis initiation
Mettl3 deletion alters expression pattern of genes involved in mouse spermatogenesis. (A) Heatmap showing the differentially expressed genes between Mettl3Ctrl and Mettl3cKO testes at P12 and their function enrichment. Two-fold expression difference and P = 0.05 as the cutoff. The enriched Gene Ontology (GO) terms of the biological process category in down or upregulated genes in Mettl3cKO samples were shown on the right panels. (B) Violin plot of expression levels of germ cell specifically expressed genes in Mettl3Ctrl and Mettl3cKO samples at P6 and P12. The log2-transformed expression level (FPKM) was used for drawing the plot. *** means the P < 10e-5, as calculated by the Wilcoxon signed-rank paired test. (C) The unsupervised hierarchical cluster analysis for the expression of germ cell specifically expressed genes in Mettl3Ctrl and Mettl3cKO samples at P6 and P12. (D) The interaction network showing genes involved in spermatogenesis regulation. The downregulated genes in Mettl3cKO testes which had been validated by qRT-PCR marked in blue. The regulation relationships were produced by pathway studio; the positive, negative regulation among these genes was marked as arrow, T-head respectively, while the regulation with unknown effects between two genes were connected directly. (E, F) qRT-PCR validation of downregulated genes involved in spermatogenesis in Mettl3cKO testes at P6 (E) and P12 (F). Student's t-test was performed and data was shown as mean ± SEM. of three independent experiments. *P < 0.05, *P < 0.01, **P < 0.001; ns, no significance.
