Figure 1
From: 5-Hydroxymethylcytosine signatures in cell-free DNA provide information about tumor types and stages
Sequencing of 5hmC in cfDNA. (A) General procedure of cell-free 5hmC sequencing. cfDNA is ligated with Illumina adapter and labeled with biotin on 5hmC for pull-down with streptavidin beads. The final library is completed by directly PCR from streptavidin beads. (B) Percentage of reads mapped to spike-in DNA in the sequencing libraries. Error bars indicate SD. (C) Metagene profiles of log2 fold change of cell-free 5hmC to input cfDNA ratio in genes ranked according to their expression in cell-free RNA-Seq.
