Figure 2
From: 5-Hydroxymethylcytosine signatures in cell-free DNA provide information about tumor types and stages
Lung cancer leads to progressive loss of 5hmC enrichment in cfDNA. (A) Genome browser view of the cell-free 5hmC distribution in a 10 mb region in chromosome 6. The overlapping tracks of healthy, non-metastatic lung cancer, metastatic lung cancer and input cfDNA samples are shown in line plot. (B) Heatmap of 1 159 metastatic lung cancer differential genes in healthy, lung cancer samples and the unenriched input cfDNA. Hierarchical clustering was performed across genes and samples. (C) Boxplot of number of hMRs (normalized to 1 million reads) identified in each group. (D) Boxplots of CCNY and PDIA6 5hmC FPKM in lung cancer and other cfDNA samples. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 1e-5, Welch t-test.
