Figure 6

Embryonic telencephalic gene expression changes due to loss of endothelial GABA and consequent postnatal phenotype. (A) Heat map showing overall top 20 differentially expressed genes in Vgat^ECKO versus Vgat^fl/fl telencephalon (n = 3). (B-D) Heat maps were further classified to show top 20 differentially expressed genes in Vgat^ECKO versus Vgat^fl/fl telencephalon in three different categories: angiogenesis (B), neurogenesis (C) and GABA neuronal development (D). (E) Validation of altered expression of angiogenesis pathway genes in E15 Vgat^fl/fl and Vgat^ECKO periventricular endothelial cells by quantitative real-time PCR. (F) A classification of genes expressed in Vgat^ECKO telencephalon using TPH1 CTD analysis shows enrichment in several neurological and psychiatric disease categories. Seizures and several different kinds of epilepsies were enriched in the list. (G-I) The scatter plots display values for each gene with signal present in tissue specimens. The percentage change in expression in Vgat^ECKO samples compared to the WT and the Tstat associated with the comparison are indicated on the axes for all genes combined (G), McTague only genes (H) and CDT genes associated with seizure conditions by marker/mechanism, marker/mechanism/therapeutic and therapeutic direct evidence (I). The color of each mark indicates the t-test result for the comparison. (J) Graphical illustration of genes with percentage change in expression in Vgat^ECKO telencephalon with respect to early infantile epileptic encephalopathy (isolated from³⁴).