Figure 1
From: Visualization of aging-associated chromatin alterations with an engineered TALE system
Conventional TALEs were insufficient to precisely track telomeres in four human tumor cell lines tested. (A-B) Co-localization analysis of telomeric FISH (red) and EGFP-TALEtelo (green) signals in the indicated human cell lines (U2OS, HeLa, HepG2, and MCF7). TALEtelo was designed using a 19-bp (A) or a 15-bp (B) telomeric repetitive sequence. Dashed lines indicate the nuclear boundaries; arrowheads indicate overlapping signals. Scale bars, 5 μm. (C) Histograms showing numbers of EGFP-TALEtelo(15 bp)-, EGFP-TALEtelo(19 bp)-, and telomeric FISH-positive nuclear dots in each cell line. n = 50 nuclei per cell line.
