Figure 4
From: Visualization of aging-associated chromatin alterations with an engineered TALE system
TTALE-mediated imaging of telomeres and centromeres in mitotic HeLa cells, hESCs, and the isogenic cell derivatives of hESCs. (A) Dynamic distribution of telomeres and centromeres at different stages of mitosis. Live HeLa cells co-expressing EGFP-TTALEcentro and mCherry-TTALEtelo were imaged at different mitotic phases. Scale bars, 5 μm. (B) Schematic diagram showing derivation of isogenic cell types from hESCs. hMSCs, hNSCs, and hVSMCs were differentiated from hESCs, and hNSCs were further differentiated into postmitotic neurons or reprogrammed into iPSCs. (C-D) Structured illumination microscopy (SIM) images showing co-localization of centromeric FISH (red) and Flag-TTALEcentro (green) (C) or telomeric FISH (red) and Flag-TTALEtelo (green) (D) signals in the indicated cell types. The number of each scatter plot represents the Pearson r value to show the linear correlation between FISH and TTALEtelo or TTALEcentro signals. Dashed lines indicate the nuclear boundary. Scale bars, 5 μm.
