Figure 8
From: Visualization of aging-associated chromatin alterations with an engineered TALE system
Lentiviral TTALE-mediated in situ telomere imaging in mouse tissues. (A) Schematic diagram of TTALE-based telomere imaging in mouse liver, anterior tibial muscle, and hippocampus. (B-D) Lentiviral EGFP-TTALEtelo-mediated telomere imaging in anterior tibial muscle (B), liver (C), and hippocampus (D) in WT mTerc+/+ and G3 mTerc−/− mice. Dye for wheat germ agglutinin (WGA), antibody against human serum albumin (anti-ALB), and antibody against NeuN (anti-NeuN) were used to label (in red) the outline of anterior tibial muscle cells (B), the cytoplasm of liver cells (C), and nuclei of the hippocampus (D), respectively. Magnified images of the boxed region are shown in the lower panels. Dashed lines indicate the nuclear boundary. Scale bars, 50 μm (wide-field images) or 2 μm (magnified images). (E) Normalized fluorescence intensities of EGFP-TTALEtelosignals in B-D. n = 100 nuclei; ***P < 0.001.
