Figure 5
From: Baf60b-mediated ATM-p53 activation blocks cell identity conversion by sensing chromatin opening
Baf60b facilitates ATM recruitment to the SWI/SNF complex and ATM activation. (A) Analyses of the interaction among Baf60b, the SWI/SNF complex, ATM, p-ATM and p-p53. The hepatic conversion was induced in TTFs transduced with HA-tagged Baf60b. Cell lysates were immunoprecipitated with an HA antibody or control IgG followed by immunoblot (IB) assays with antibodies against the indicated proteins. (B, C) 3TF-transduced TTF lysates were immunoprecipitated with antibodies against endogenous Baf60b (B) or against endogenous Brg1 (C) followed by IB analyses with antibodies against indicated proteins. (D) Baf60b was silenced by sh1-Baf60b in TTFs. Forty-eight hours after 3TF transduction, lysates of TTFs were immunoprecipitated with Brg1 antibody followed by IB analysis with ATM and p-ATM antibodies.
