Figure 6
From: Baf60b-mediated ATM-p53 activation blocks cell identity conversion by sensing chromatin opening
The NLS and SWIB domains are necessary for Baf60b-dependent ATM activation. (A) HA-tagged Baf60b and two Baf60b mutants (ΔNLS and ΔSWIB) were individually transduced into TTFs. Interactions among Baf60b mutants, Brg1, ATM and p-ATM were determined. (B-D) ΔNLS, ΔSWIB and HA-tagged Baf60b were transduced into Baf60b-silenced TTFs in which the endogenous 3′UTR of Baf60b mRNA was targeted by sh2-Baf60b. 3TF-induced p-ATM was analyzed by immunofluorescence staining and quantified by LAS AF Lite; n = 6 cells were analyzed for each group (B). p-p53 (Ser15) protein level was determined by the western blot assay (C) and iHep colony numbers were quantified 8 days after iHep induction; n = 4 independent experiments (D). (E) A schematic model showing that a repressive mechanism involving Baf60b-mediated ATM activation senses chromatin opening and limits hepatic conversion. Error bars indicate SD. *P< 0.05. Student's t-test.
