Figure 5
From: Intracellular HMGB1 as a novel tumor suppressor of pancreatic cancer
Inhibition of nuclear HMGB1 loss and release by glycyrrhizin limits K-Ras-induced PanIN formation. (A, B) Effects of K-RasG12D transfection on HMGB1 expression (A) and release (B) in primary mouse pancreatic acinar cells (n = 3, ***P < 0.001, data are expressed as means ± sem, unpaired t-test). (C, D) Effects of glycyrrhizin (500 μM) on HMGB1 expression (C) and release (D) in primary mouse pancreatic acinar cells after K-RasG12D transfection for 48 h (n = 3, ***P < 0.001, data are expressed as means ± sem, unpaired t-test). (E-G) Effects of glycyrrhizin (10 mg/kg) on PanIN formation (E) and HMGB1 expression (F) and release (G) on day 21 in a cerulein-mediated accelerated oncogenic K-Ras mouse model of pancreatic cancer (n = 5 mice/treatment, ***P < 0.001, data are expressed as means ± sem, unpaired t-test).
